How do you prepare samples for HPLC?
The simplest form of sample preparation is direct injection of the analyte into the HPLC system. Even if this is possible there will be sample preparations required unless the analyst can sample directly from the sam- ple container, which is rare.
What is Solid Phase Extraction PDF?
Solid-phase extraction (SPE) is a sample preparation technique routinely used in analytical laboratories for the extraction of analytes from a complex matrix. This sample preparation technique enables the extraction, cleanup and concentration of analytes prior to their quantification.
What is solid phase microextraction sampling?
SPME is a sampling technique based on absorption developed by Arthur and Pawliszyn. With SPME, the analytes are absorbed from the liquid or gaseous sample on to an absorbent coated fused silica fibre, which is part of the syringe needle, for a fixed time.
What is the mobile phase in HPLC?
The solvent used to separate components in a liquid sample for HPLC analysis is called the mobile phase. The mobile phase is delivered to a separation column, otherwise known as the stationary phase, and then to the detector at a stable flow rate controlled by the solvent delivery pump.
What is microwave-assisted method?
Microwave-assisted synthesis works on the basis of aligning dipoles of the material in an external field via the excitation produced by microwave electromagnetic radiations and is usually executed in combination with a known synthesis strategy.
Why is SPE used?
Common applications of SPE Pharmaceutical compounds and metabolites in biological fluids. Drugs of abuse in biological fluids. Environmental pollutants in drinking and wastewater. Pesticides, antibiotics or mycotoxins in food/agricultural matrices.
What is the difference between SPE and SPME?
The principal difference is that SPE is carried out with a relatively large sorptive wafer the size of a small filter paper and requires post-sorption liquid-phase extraction of analytes; SPME is accomplished with a small fibre or tube coated with sorptive material.
Why acetonitrile is used in HPLC?
Acetonitrile is often used because of its low UV cutoff, lower viscosity (methanol forms highly viscous mixtures with water at certain concentrations), and higher boiling point.