What is dithiobis succinimidyl propionate?
DSP (Dithiobis(succinimidyl Propionate)) DSP is a water-insoluble, homobifunctional N-hydroxysuccimide ester. DSP, a protein crosslinker, is thiol-cleavable, primary amine-reactive and has been used in many applications.
What is DSP chemistry?
Disodium Hydrogen Phosphate (DSP) : (Na2HPO4) Acetophenone (2,4-Dinitrophenyl) Hydrazone.
What is DSP crosslinking?
Recommendations. Thermo Scientific Pierce DSP (Lomant’s Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm.
What do crosslinkers do?
Cross-linker increases the viscosity of gelling agents by connecting the separate gel polymers together. Cross-linker significantly increases the viscosity of linear gel by increasing the molecular weight of the base polymer by linking multiple molecules together.
What is DSP protocol?
Overview. Delphix Session Protocol, or DSP, is a communication protocol that operates at the session and presentation layer in the OSI model. DSP is officially registered with the Internet Assigned Numbers Authority under the service name of dlpx-sp and port number 8415.
Does crosslinking increase brittleness?
And excessive crosslinking initiated in the melted polymer can result in brittle materials that lack crystalline regions—regions that also contribute to valuable mechanical properties.
What is a crosslinking agent?
Crosslinking Agents. Crosslinking is the formation of chemical links between molecular chains to form a three-dimensional network of connected. molecules. The vulcanization of rubber using elemental sulfur is an example of crosslinking, converting raw rubber from a weak plastic to a highly resilient elastomer.
What is the major purpose of cross-linking in polymers?
Introduction. Chemical cross-linking has been widely used to alter the physical properties of polymeric materials, the vulcanization of rubber being a prototypic example. Linking of polymer chains through chemical linkages gives a material a more rigid structure and potentially a better-defined shape.
How do crosslinking agents work?
Crosslinking is the process of chemically joining two or more molecules by a covalent bond. Crosslinking reagents (or crosslinkers) are molecules that contain two or more reactive ends capable of chemically attaching to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules.
Which is the most commonly used agent for crosslinking of enzymes?
Among these, glutaraldehyde probably represents the most commonly used crosslinking reagent (Migneault et al. 2004). Additionally, there are a few examples of multifunctional crosslinkers that can be used to target proteins at more than two functional groups simultaneously.
What are examples of cross-linked polymers?
Examples of cross-linked polymers include: Polyester fiberglass, polyurethanes used as coatings, adhesives, vulcanized rubber, epoxy resins and many more.
What is crosslinking used for?
Crosslinking reagents are used in a variety of techniques to assist in determining partners and domains of protein interactions, three-dimensional structures of proteins, and molecular associations in cell membranes.
How much does a DSP cost?
The National Association of DSPs estimates the cost of replacing a DSP is between $2413 and $5200.
What are cross-linking agents?
What is cross-linking reagents?
Crosslinking reagents (or crosslinkers) are molecules that contain two or more reactive ends capable or chemically attaching to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. This article describes the chemistry and variety of crosslinkers that exist.
What are cross linking agents?
Which has cross-linked polymer chains?
Bakelite has cross-linked polymer chains…