What is the significance of using blue dextran in molecular exclusion chromatography when is it used and why?
Blue dextran is also used for checking the column packing. A symmetrical peak of elution indicates homogeneity of packing. The inner volume (Vi) of the column can be determined by subtracting the void volume from the elution volume of small molecules such as glucose or tyrosine having K d — 1.0.
What Colour is blue dextran?
Blue Dextran is a high-molecular-weight glucose polymer (original mol wt 2 x 10(6) g/mol) containing covalently bonded Reactive Blue 2 dye (approximately mmol/g dextran). This blue dye is known for its high binding affinity to a wide variety of proteins, with a particularly high affinity for serum albumin.
What is the function of dextran?
Dextran is a medication used in managing and treating various clinical conditions, including during hemorrhage, shock, surgical procedures, radiological imaging, antithrombotic administration, and ophthalmic relief of xerophthalmia.
What type of solution is dextran?
Dextran produces relatively low-viscosity solutions, which distinguishes it from other high-molecular-weight polysaccharides. It is a neutral polymer.
Is dextran water soluble?
Dextran is a natural branched bacterial polysaccharide produced by Leuconostoc mesenteroides that consists of α-1,6-d-glucopyranose with α-1,2, α-1,3, or α-1,4 linked side chains (Jiang et al., 2004). It is soluble in water and organic solvents.
How do you precipitate dextran?
DEXTRANS AND DEXTRAN DERIVATIVES WHICH ARE READILY SOLUBLE IN COLD WATER ARE PRODUCED BY ADDING A SIRUPY ALCOHOL- OR KETONE-WATER MIXTURE OF DEXTRAN OR A DEXTRAN DERIVATIVE TO A HIGHLY CONCENTRATED ALCOHOL OR KETONE TO PRODUCE A GRANULAR PRECIPITATE WHICH IS WASHED WITH THE RESPECTIVE CONCENTRATED ALCOHOL OR KETONE.
Which method is used for fractionation of dextran?
Method for the fractionation of dextran by centrifugal precipitation chromatography.
How do you make dextran solution?
Making Up a 10% Dextran Solution:
- To make 40 mL, measure out 4 grams of Dextran (40,000 MW, Sigma #31389) in a 50 mL conical vial.
- Add sterile water up to 40 mL and then dissolve the dextran, using the vortex mixer.
- Once dissolved, the total volume will be less than 40 mLs so add water to bring the volume up to 40 mL.