What is the molecular weight of beta-actin?
approximately 42 kD
Beta-Actin Molecular Weight The molecular weight (MW) of beta-actin is approximately 42 kD.
How many kDa is beta-actin?
42 kDa
Though beta-actin has a molecular weight of 42 kDa, degradation by apoptosis will produce 38-41 kDa fragments on a Western blot.
How many BP is beta-actin?
142,892,509 bp
Beta-actin
Gene location (Mouse) | ||
---|---|---|
Chr. | Chromosome 5 (mouse) | |
Band | 5 G2|5 81.8 cM | 142,888,870 bp |
142,892,509 bp |
What is B actin mRNA?
Beta-actin mRNA is localized to the leading lamella in chicken embryo fibroblasts (CEFs) and several other cell types, just proximal to the lamellipodia (1–4). Localization of β-actin mRNA depends on an intact actin cytoskeleton in CEFs (5).
What is the molecular weight of myosin?
The myosin molecule is composed of two subunits of molecular weight 200,000 and several small polypeptides ranging in molecular weight from 15,000 to 25,000 daltons [6].
What is the MW of GAPDH?
36 kDa
GAPDH molecule is a homotetramer composed of 36 kDa subunits. Thus the molecular weight for the whole molecule is 144 kDa.
Why does B actin make a good loading control?
Beta-Actin (42 kDa) is commonly chosen as a loading control due to its general expression across all eukaryotic cell types. The expression levels of this protein do not vary drastically due to cellular treatment, which is another reason the protein makes a suitable control.
How many base pairs is actin?
A 40-base-pair sequence in the 3′ end of the beta-actin gene regulates beta-actin mRNA transcription during myogenesis.
What is beta-actin used for?
Beta actin specifically plays a crucial role in other relating functions like; cell migration, involved in pathways at the blood-brain barrier, cell division, muscle contraction, cell mobility, secretion, phagocytosis and gene expression.
How and why does β actin mRNA target?
We postulate that delocalization of beta-actin mRNA results in delocalization of nucleation sites and beta-actin protein from the leading edge followed by loss of cell polarity and directional movement. Hence the physiological consequences of beta-actin mRNA delocalization affect the stability of the cell phenotype.
What does B actin do?
How many kDa is myosin?
223 kDa
Myosin is a hexameric protein consisting of two myosin heavy chains (MHCs, ~223 kDa), two pairs of regulatory light chains (RLCs, ~19 kDa) and two pairs of essential light chains (ELCs, ~22 kDa) (Figure 1a).
Why is beta actin a loading control?
Is beta-actin a protein?
ACTB Gene – Actin Beta Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed.
Why does β actin make a good loading control?
Is beta-actin expressed in muscle?
Four of these isoforms are expressed primarily in striated (αsk and αca) and smooth (αsm and γsm) muscle cells, whereas the two cytoplasmic β-actin and γ-actin isoforms are ubiquitously expressed.
Is beta actin a protein?
Beta Actin is one of six Isomers of the actin protein. It is a major cytoskeletal filament protein found in eukaryotes, prokaryotes and fungi.
What is beta actin housekeeping gene?
β-actin is a cytoskeletal protein involved in cell structure and motility. Based on these basic and ubiquitous cellular functions, GAPDH and β-actin are considered as housekeeping genes.
What is the molecular weight of myosin in kDa?
220 kDa
Skeletal muscle myosin consists of two heavy chains of molecular weight 220 kDa each and two pairs of light chains that have molecular weights in the range 15-22 kDa [6,7].
How many myosin molecules are in a thick filament?
300 myosin molecules
The bipolar thick filament is formed of approximately 300 myosin molecules and their associated proteins (Craig & Offer, 1976; Luther, Munro, & Squire, 1981). It has a strictly uniform dimension of 1.6 µm in length and 15 nm in diameter, which is independent of the type of muscle fiber (Huxley, 1963).
How many base pairs is GAPDH?
In order to understand the mechanism of this regulation, we have isolated and sequenced 5.4 kilobase pairs of a 12-kilobase pair human genomic clone encoding a functional GAPDH gene. The gene consists of 9 exons and 8 introns with eukaryotic signals necessary for the transcription and translation of GAPDH mRNA.
What is the function of actin b?
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371].
What is the role of actin in the interaction between Enos and actin?
An eight-amino acid residue (326-333) in actin has been shown to mediate the interaction between actin and eNOS Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma.
Does actin cleavage accelerate proteolysis in skeletal muscle?
Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (5-7). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (7). Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.
Does 8h10d10 cross-react with β actin?
β-Actin (8H10D10) Mouse mAb detects endogenous levels of total β-actin protein. Due to the high sequence identity between the cytoplasmic actin isoforms, β-actin and cytoplasmic γ-actin, this antibody may cross-react with cytoplasmic γ-actin. It does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms.