How do you make a BCG solution?
Prepare the Bromocresol Green Solution
- Dissolve 0.1 g of bromocresol green in 75 mL of ethyl alcohol.
- Dilute the solution with ethyl alcohol to make 100 ml.
What is the chemical formula for bromocresol green?
C21H14Br4O5SBromocresol green / Formula
How do you make bromocresol green methyl red indicator?
Methyl Red-Bromcresol Green Indicator: Dissolve 0.33 g bromcresol green and 0.66 g methyl red dyes in 1 liter of 95% ethyl alcohol. Add sufficient 0.1 N sodium hydroxide solution to produce a green color; add dropwise just sufficient 0.1 N hydrochloric acid solution to produce a deep wine-red color.
What is the pH of bromocresol green?
pH 3.8 to 5.4
Bromocresol green (BCG) (3′, 3”, 5′, 5”-tetrabromo-m-cresolsulfonphthalein) (Fig. 1b) is a sulfonphthalein dye, with a transition range of pH 3.8 to 5.4.
How do you calculate the BCG matrix?
How To Make A BCG matrix?
- Relative Market Share = Product’s sales this year/Leading rival’s sales this year.
- Market growth rate is usually given by: (Product’s sales this year – Product’s sales last year)/Product’s sales last year.
What is the BCG method?
The method is based on the specific binding of bromocresol green (BCG), an anionic dye, and the protein at acid pH produce a color change of the indicator from yellow –green to green –blue with the resulting shift in the absorption wavelength of the complex.
How do you make a mixed indicator solution?
Preparation of Methyl Red-Methyl Blue Indicator (Mixed Indicator)
- – Weigh out 0.1 g of methyl red (as sodium salt) and dissolve completely in 50 ml of ethanol (ethyl alcohol).
- – Weigh out 0.05 g of methylene blue and dissolve completely in 50 ml of water.
How does bromocresol green indicator work?
In aqueous solution, Bromocresol green will ionize to give the monoanionic form (yellow), that further deprotonates at higher pH to give the dianionic form (blue), which is stabilized by resonance. It becomes yellow at acidic pH level (pH 3.8) and Blue green at from pH 5.4.
How do you make a 0.04 Bromothymol blue solution?
Dissolve bromthymol blue in NaOH. Dilute to 500 ml with distilled water….BAM R10: 0.04% Bromthymol Blue Indicator.
| Bromthymol blue | 0.2 g |
|---|---|
| 0.01 N NaOH | 32 ml |
At what pH does bromocresol green change color?
3.8-5.4
According to the table, bromcresol green, has a pH range for color change of 3.8-5.4 and the color change is yellow to blue. Therefore, a solution whose pH is less than 3.8 will have a yellow color with bromcresol green indicator. On the other hand, a solution whose pH is higher than 5.4 will have a blue color.
How do you calculate BCG industry growth rate?
It can also be calculated by looking at average revenue growth of the leading industry firms. Market growth rate is measured in percentage terms. The midpoint of the y-axis is usually set at 10% growth rate, but this can vary. Some industries grow for years but at average rate of 1 or 2% per year.
How do you calculate albumin?
Capillary zone electrophoresis (CZE) is successfully used for albumin estimation. A narrow bore capillary column in a liquid medium is used to separate albumin from other serum proteins by applying high voltage current. This technique provides more specific results for albumin estimation compared to BCG [68].
How do you prepare a laboratory solution?
Dissolve 93.52 g of NaCl in about 400 mL of distilled water, then add more water until final volume is 800 mL. If starting with a solution or liquid reagent: When diluting more concentrated solutions, decide what volume (V2) and molarity (M2) the final solution should be.
What is the composition of mixed indicator?
A mixture of 3parts of phenolphthalein and 1 part of naphtholphthalein shows a change in color from pale rose to violet at pH = 8.9. The mixed indicator is useful for the titration of phosphoric acid to diproti stage.
What color is bromocresol green in basic solutions?
1 Answer. In aqueous solution, bromocresol green will ionize to give the monoanionic form (yellow), that further deprotonates at higher pH to give the dianionic form (blue), which is stabilized by resonance: The acid dissociation constant (pKa) of this reaction is 4.8.
What is BCG growth rate?
The market growth rate varies from industry to industry but usually shows a cut-off point of 10% – growth rates higher than 10% are considered high, while growth rates lower than 10% are considered low. Learn more about strategy in CFI’s Business Strategy Course.
How do you prepare a BCG Matrix?
How To Make A BCG matrix?
- Step 1: Choose the product. BCG matrix can be used to analyse Business Units, separate brands, products or a firm as a unit itself.
- Step 2: Define the market.
- Step 3: Calculate the relative market share.
- Step 4: Find out the market growth rate.
- Step 5: Draw the circles on a matrix.
How do you calculate market growth rate?
Calculate Market Growth Rate Calculate market growth by subtracting the market size for year one from the market size for year two. Divide the result by the market size for year one and multiply by 100 to convert to a percentage.
What is the principle of estimation of albumin by BCG method?
PRINCIPLE. Albumin is known for its ability to bind many types of organic compounds, including organic dyes. When albumin binds with Bromcresol Green (BCG) it causes a change in the absorbance maximum of BCG. This change can be measured spectrophotometrically and used to determine albumin concentration.
What are the steps for preparing solution?
From solid material Determine the concentration and amount of solution required for the experiment. Calculate the amount of solute required to prepare the desired solution. Weigh out the amount of solute calculated in step (2) and obtain a volumetric flask of appropriate volume. Add the solute to the volumetric flask.
How do you calculate the volume needed to make a solution?
Strategy:
- Calculate the number of moles of glucose contained in the indicated volume of dilute solution by multiplying the volume of the solution by its molarity.
- To determine the volume of stock solution needed, divide the number of moles of glucose by the molarity of the stock solution.