What are the advantages of disposable bioreactor?
While the term “disposable” often connotes wastefulness, bioreactors that use disposable bags instead of traditional culture vessels actually have significant advantages when it comes to resource conservation, including cost savings, environmental sustainability, and improving safety and compliance.
What is disposable reactor?
A single-use or disposable bioreactor is a bioreactor with a disposable bag instead of a culture vessel. Single-use bioreactors allow processors to implement the use of disposable technology, such single-use bags, in manufacturing process steps that had previously been reserved for stainless steel equipment.
What are single-use bioreactors used for?
Pharmaceutical companies use single-use bioreactors for media and buffer preparation, cell harvesting, filtration, purification, and virus inactivation.
What are the cons of single-use bioreactor technology?
Disadvantages:
- Limitation in liquid transfer.
- Scalability is an issue: larger-scale bioreactor bags are needed than are available.
- Expensive to use:repetitive purchases required.
- Performance is not completely proven: new technology.
- Slight increase in variable costs per run.
What are disposable technologies?
Initially, disposable technologies were designed as alternatives to reusable consumables and equipment as a means of eliminating tedious cleaning processes. Today, they are resilient competitors to traditional biomanufacturing equipment and essential parts of bioprocessing.
What are the advantages of using a bioreactor?
A bioreactor has several advantages including constant regulation of conditions during operation, enhanced nutrient uptake system, and easy handling of large quantities of cultures. Bioreactors are generally more time and cost effective than other culture systems.
How does an airlift reactor work?
An airlift bioreactor works by agitating the contents of the bioreactor pneumatically using gas. The gas used for agitation can act to either, introduce new molecules to the mixture inside of the bioreactor, or remove specific metabolic molecules produced by microorganisms.
Are bioreactors possible?
Commercial single-use bioreactors have been available since the end of the 1990s and are now made by several well-known producers (See below) .
How much does a single-use bioreactor cost?
Cost Per Month Graph Values
| Single-Use System | Cost Per Month | |
|---|---|---|
| Operating Costs (Single-Use) | Media | $1,200 |
| Rent | $2,800 | |
| Monthly Operating Costs Sub-total | $46,133 | |
| Total | Cost Per Month | $67,675 |
What is single-use technology in pharmaceutical manufacture?
Single-use systems (SUS) refers to biopharmaceutical manufacturing (bioprocessing) equipment designed to be used once (or for a single manufacturing campaign) and then discarded. Generally, SUS equipment is composed primarily of plastic components that have been sealed and sterilized using gamma irradiation.
How has the development of bioreactor helped in biotechnology?
The bioreactors are of great use in biotechnology in numerous ways. The process of production using bioreactor provides a control system especially over temperature, pH as well as the pressure of the system. Other important functions required for the process can be easily measured and controlled.
Why is airlift bioreactor used?
Airlift bioreactors are used for cell culturing, pallet form fermentation, and immobilized enzyme reactions. Typically, airlift bioreactors are used when the desired reactants and/or final products are in a gaseous state and for aerobic cell cultures.
What are the advantages and disadvantages of airlift fermenter?
Principle of an airlift bioreactor. Advantage: Only compressed air (or other gas) required, low shear forces. Disadvantage: Relatively high costs for compressing flow rate, difficult to adjust.
Why are bioreactors important?
Bioreactors ensure cell survival through adequate delivery of essential nutrients throughout the three-dimensional tissue engineered construct. Bioreactors can also guide tissue structure, organization, and ultimately function through the application of chemical and mechanical stimuli.
Why are bioreactors so expensive?
In general, the cost of laboratory space, infrastructure and staff exceed by far the initial acquisition costs of a bioreactor. Which in the end justifies the truth that quality matters and comes with a price.
What industries use bioreactors?
By end user, the pharmaceutical and biopharmaceutical companies segment accounted for the largest share of the single-use bioreactors market in 2020. Based on end users, the market is segmented into pharmaceutical and biopharmaceutical companies, CROs & CMOs, and academic and research institutes.
Where are bioreactors used?
Bioreactors are used in the food, medical, pharmaceutical and cosmetics industries. Each of these produces certain types of biomolecules, depending on the use of the final product. Some are also produced in the form of complex products, while others remain molecules.
What is the goal of bioreactors?
A bioreactor is a vessel for providing a controlled environment for cells to grow under specific conditions. In bioreactors, we provide cell metabolites and agitation in order to direct cell growth and activity in specific ways, and to harvest the cells or products they have been engineered to produce.
What are the downsides of using single-use tools?
The Disadvantages of Single-Use Technologies
- Difficulty in efficiently producing in bulk.
- Having to rely on vendors.
- Higher consumables cost.
- Inventory storage.
- Lot/material tracking.
- Vendor-initiated change controls.
- Limited number of gamma irradiation facilities.
What are single-use technologies?
Single-use technologies include tubing, capsule filters, ion exchange membrane chromatography devices, mixers, bioreactors, product holding sterile bags in place of stainless steel vessels (sterile fluid containment bags), connection devices and sampling receptacles.
What are disposable bioreactors and how do they work?
Disposable bioreactors, buffer make-up and hold tanks, and attachments are gaining in popularity because they can reduce risk of cross-contamination between batches while providing flexibility, minimizing turnaround time, reducing cleaning costs, and easing validation restrictions.
What’s new in single-use bioreactors?
New single-use bioreactor designs are emerging in the market utilizing stirred agitation methods for effective oxygen transfer and cultivation of both cell culture and microbial culture processes.
What are the most successful bioreactors?
The most successful of the disposable bioreactors is the wave-type bioreactor that was introduced about 10 years ago [41]. The cells are cultivated in disposable plastic bags of volumes of up to 500 l mounted on a rocking table.
Which bioreactors are suitable for which cell types?
Bioreactors are appropriate for both nonadherent and adherent (with the addition of microcarriers) cell types. Disposable bioreactors are especially suitable for personalized medicine operations such as autologous cell therapy.