What is an anti HA antibody?
The HA (hemagglutinin) tag is derived from the human influenza virus HA protein. It is well characterized and is used extensively as a general antibody epitope tag. HA tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
What is a DDK tag?
Target Information. DYKDDDDK Tag (FLAG® tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology.
What does T7 primer do?
The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications2.
What does T7 polymerase do?
T7 RNA polymerase is a very active enzyme: it synthesizes RNA at a rate several times that of E. coli RNA polymerase and it terminates transcription less frequently; in fact, its transcription can circumnavigate a plasmid, resulting in RNA several times the plasmid length in size.
Why is T7 promoter used?
What does the T7 tag do?
The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. 6 Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
What is AviTag?
The AviTag™ is a popular fusion tag due to its powerful and versatile properties. Fused to your protein, the AviTag™ provides a multi-functional system useful for many applications including: Expression. Imaging. Detection.
How does T7 work?
What T7 means?
Abstract. The late bacteriophage T7 terminator (T7-T phi) encodes an RNA sequence that can form a stable stem-loop structure followed by a run of six uridylate residues; termination occurs at a 3′ G residue just downstream of the U run.
How does T7 polymerase work?