What is FITC in annexin V?
Annexin V-FITC Apoptosis Staining / Detection Kit ab14085 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
What is Annexin V staining?
Annexin V staining is a common method for detecting apoptotic cells. Thermo Fisher Scientific offers high-quality fluorescent annexin V conjugates as standalone reagents and in a variety of kits for use in flow cytometry and for imaging suspension cells.
How does annexin V assay work?
Principles of the Annexin V Apoptosis Assay: exposed on the outside surface of the plasma membrane. Because the anticoagulant protein Annexin V binds with high affinity to phosphatidylserine, fluorochrome-conjugated Annexin V can been used to detect apoptotic cells by flow cytometry.
What is BSA staining?
Description. PharmingenStain Buffer (BSA) can be used for the immunofluorescent staining of single-cell suspensions prepared from either lymphoid tissues, bone marrow, peripheral blood, or cultured cells.
Does PE bleed into FITC?
where FITC bleeds into the PE channel and PE bleeds back into FITC. To correct for spectral overlap during multicolor flow cytometry experiments, color compensation must be performed. The goal of color compensation is to correctly quantify each dye with which a particular cell is labeled.
Can I use FITC to compensate GFP?
Caveats: Just because GFP, CFSE and FITC are detected in the same channel (FL1), it does not mean you can use them interchangeably when setting up your compensation. GFP, CFSE and FITC are unique flourochromes and have different spillover into other channels.
Can you use FITC and PE together?
FITC overlap a lot with Pe, don’t use them together on same cells.
What is anti annexin?
This assay is useful in the diagnosis of primary or secondary antiphospholipid syndrome (APS). Annexin V antibodies are found in patients with APS, SLE, or other collagenoses, as well as those with rheumatoid arthritis.
Does FITC overlap with PE?
In some experiments FITC may be combined with other dyes, for example PE, that emit yellow and orange photons. In those cases the relative contribution of each fluorophore to the signal in a given detector must be determined (Figure 11).